SiRNA Validation Data

siRNA ID:

120719

Gene ID:

Transcript(s):

NM_001040668.1 NM_001282516.1 NM_001282517.1 NM_001282519.1 NM_001282520.1 NM_138639.1 NR_104200.1 NR_104201.1 NR_104202.1 NR_104203.1 NR_104204.1 XM_017027345.1 XM_017027346.1

Validation Results:

24%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00369327_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.