93
NM_001221.3
NM_001321566.1
NM_001321567.1
NM_001321568.1
NM_001321569.1
NM_001321570.1
NM_001321571.1
NM_001321572.1
NM_001321573.1
NM_001321574.1
NM_001321575.1
NM_001321576.1
NM_001321577.1
NM_001321578.1
NM_001321579.1
NM_001321581.1
NM_001321583.1
NM_001321584.1
NM_001321585.1
NM_001321586.1
NM_001321587.1
NM_001321588.1
NM_001321589.1
NM_001321590.1
NM_001321591.1
NM_172114.1
NM_172115.2
NM_172127.2
NM_172128.2
XM_005263253.3
XM_005263254.3
XM_006714330.2
XM_011532292.2
XM_017008672.1
XM_017008673.1
XM_017008674.1
XM_017008675.1
XM_017008676.1
XM_017008677.1
XM_017008678.1
8%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.