981
NM_015533.3
XM_011544912.2
XM_017017517.1
XM_017017518.1
XM_017017519.1
XM_017017520.1
XM_017017521.1
XM_017017522.1
XM_017017523.1
XM_017017524.1
9%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.