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SiRNA Validation Data

siRNA ID:

s24920

Gene ID:

26007

Transcript(s):

NM_015533.3 XM_011544912.2 XM_017017517.1 XM_017017518.1 XM_017017519.1 XM_017017520.1 XM_017017521.1 XM_017017522.1 XM_017017523.1 XM_017017524.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00210137_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.