103592
NM_001322227.1
NM_001322228.1
NM_001322229.1
NM_001322230.1
NM_001322231.1
NM_001322233.1
NM_001322234.1
NM_001322235.1
NM_001322236.1
NM_001322237.1
NM_004755.3
NM_182398.2
XM_017021786.1
8%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00178054_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.