SiRNA Validation Data

siRNA ID:

105256

Gene ID:

Transcript(s):

NM_001032730.1 NM_032236.5 XM_005246009.2 XM_006710951.2 XM_006710955.3 XM_011542262.2 XM_011542263.2 XM_011542264.1 XM_011542265.2 XM_011542267.2 XM_017002495.1 XM_017002496.1

Validation Results:

8%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00218139_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.