112262
NM_032482.2
XM_005259659.3
XM_005259660.3
XM_011528359.2
XM_011528360.1
XM_017027366.1
21%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00287200_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.