SiRNA Validation Data

siRNA ID:

119550

Gene ID:

Transcript(s):

NM_000249.3 NM_001167617.1 NM_001167618.1 NM_001167619.1 NM_001258271.1 NM_001258273.1 NM_001258274.1 XM_005265161.1 XM_005265163.1 XM_005265164.1 XM_005265166.1 XM_011533727.1 XM_017006449.1 XM_017006450.1 XM_017006451.1

Validation Results:

25%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00179866_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.