122221
NM_001318509.1
NM_001318510.1
NM_004458.2
NM_022977.2
XM_005262109.2
XM_006724635.2
XM_011530888.2
XM_011530889.2
17%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00244871_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.