SiRNA Validation Data

siRNA ID:

136339

Gene ID:

Transcript(s):

NM_001184970.1 NM_001184971.1 NM_007229.3 XM_005261319.3 XM_011529846.1 XM_011529847.2 XM_011529848.2 XM_011529849.2 XM_011529850.2 XM_011529851.2 XM_011529852.1 XM_011529853.1 XM_017028562.1 XM_017028563.1 XM_017028564.1 XM_017028565.1 XM_017028566.1 XM_017028567.1 XM_017028568.1

Validation Results:

16%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00200589_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.