213303
NM_001278554.1
NM_001278555.1
NM_006357.3
NM_182678.2
XM_005246244.2
XM_011510492.2
XM_017003166.1
XM_017003167.1
NR_110506.1
8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00818954_mH]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.