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SiRNA Validation Data

siRNA ID:

2453

Gene ID:

1312

Transcript(s):

NM_000754.3 NM_001135161.1 NM_001135162.1 NM_007310.2 XM_011529886.1 XM_017028594.1 XM_017028595.1

Validation Results:

7%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00241349_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.