43464
NM_001003794.2
NM_001256585.1
NM_007283.6
XM_011512377.1
XM_011512378.1
XM_011512379.2
XM_011512383.2
XM_017005662.1
XM_017005663.1
XM_017005664.1
XM_017005666.1
19%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00200752_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.