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SiRNA Validation Data

siRNA ID:

581

Gene ID:

9252

Transcript(s):

NM_001322227.1 NM_001322228.1 NM_001322229.1 NM_001322230.1 NM_001322231.1 NM_001322232.1 NM_001322233.1 NM_001322234.1 NM_001322235.1 NM_001322236.1 NM_001322237.1 NM_004755.3 NM_182398.2 XM_017021786.1

Validation Results:

8%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.