6310
NM_001227.4
NM_001267056.1
NM_001267057.1
NM_001320911.1
NM_033338.5
NM_033339.4
NM_033340.3
XM_006718017.3
XM_017016763.1
XM_017016764.1
17%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00169152_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.