s10369
NM_000289.5
NM_001166686.1
NM_001166687.1
NM_001166688.1
XM_005268974.1
XM_005268975.1
XM_005268976.3
XM_005268977.1
XM_005268978.2
XM_005268979.1
XM_011538487.1
XM_011538488.2
XM_017019467.1
XM_017019468.1
XM_017019469.1
6%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00175997_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.