s11180
NM_002758.3
XM_005257515.2
XM_005257516.2
XM_006721975.3
XM_011525025.2
XM_011525026.2
XM_011525027.2
11%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177150_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.