SiRNA Validation Data

siRNA ID:

s11244

Gene ID:

5636

Transcript(s):

NM_001243936.1 NM_001243940.1 NM_001243941.1 NM_001243942.1 NM_002767.3 XM_005256725.2 XM_005256726.1 XM_005256729.4 XM_017024864.1 XM_017024865.1 XM_017024866.1 XM_017024867.1 XM_017024868.1 XM_017024869.1 XM_017024870.1 XM_017024871.1 XM_017024872.1 XM_017024873.1 XM_017024874.1 XM_017024875.1 XM_017024876.1

Validation Results:

19%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00160482_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.