s12280
NM_004586.2
XM_005274573.2
XM_005274577.2
XM_006724507.3
XM_011545555.1
XM_011545556.1
XM_011545557.2
XM_011545558.2
XM_011545559.1
XM_011545560.1
XM_011545561.1
XM_011545562.1
XM_011545563.2
XM_017029713.1
XM_017029714.1
XM_017029715.1
XM_017029716.1
XM_017029717.1
XM_017029718.1
XM_017029719.1
4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177936_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.