s160
NM_001012732.1
NM_001921.2
XM_005262778.3
XM_005262779.3
XM_005262780.3
XM_005262781.4
XM_005262782.3
XM_006714115.3
XM_006714116.3
XM_011531674.2
XM_011531675.2
XM_011531676.2
XM_017007816.1
XM_017007817.1
XM_017007818.1
XM_017007819.1
XM_017007820.1
XM_017007821.1
XM_017007822.1
XM_017007823.1
XM_017007824.1
11%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00157241_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.