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SiRNA Validation Data

siRNA ID:

s16460

Gene ID:

8648

Transcript(s):

NM_003743.4 NM_147223.2 NM_147233.2 XM_005264625.1 XM_005264626.1 XM_005264628.1 XM_017005168.1 XM_017005169.1

Validation Results:

19%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00186661_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.