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SiRNA Validation Data

siRNA ID:

s2423

Gene ID:

840

Transcript(s):

NM_001227.4 NM_001267056.1 NM_001267057.1 NM_001267058.1 NM_001320911.1 NM_033338.5 NM_033339.4 NM_033340.3 XM_006718017.3 XM_011540260.1 XM_017016763.1 XM_017016764.1

Validation Results:

4%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00169152_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.