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SiRNA Validation Data

siRNA ID:

s39830

Gene ID:

85007

Transcript(s):

NM_001278346.1 NM_153373.3 NR_103508.1 NR_103510.1 XM_006714925.2 XM_011534669.2 XM_011534671.2 XM_011534672.2 XM_011534674.2 XM_017009995.1 XM_017009996.1 XM_017009997.1 XM_017009998.1 XM_017009999.1

Validation Results:

18%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00538025_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.