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SiRNA Validation Data

siRNA ID:

s591

Gene ID:

1540

Transcript(s):

NM_001042355.1 NM_001042412.1 NM_015247.2 XM_005255812.2 XM_006721149.1 XM_011522907.2 XM_011522908.1 XM_011522911.2 XM_017022977.1 XM_017022978.1 XM_017022979.1 XM_017022980.1 XM_017022981.1

Validation Results:

19%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00211000_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.