SiRNA Validation Data

siRNA ID:

103323

Gene ID:

Transcript(s):

NM_001145443.2 NM_001282630.2 NM_001314063.1 NM_001323016.1 NM_001323017.1 NM_004566.3 NR_136554.1 XM_005252464.1 XM_011519493.1 XM_017016325.1 XM_017016326.1 XM_017016327.1 XM_017016328.1 XM_017016329.1

Validation Results:

15%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00190079_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.