105118
NM_001128610.2
NM_001283049.1
NM_005154.4
XM_006720761.3
XM_006720762.3
XM_011522193.2
XM_017022718.1
XM_017022719.1
XM_017022720.1
XM_017022721.1
XM_017022722.1
10%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00987109_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.