SiRNA Validation Data

siRNA ID:

105914

Gene ID:

Transcript(s):

NM_001012644.2 NM_001320478.1 NM_001320479.1 NM_080611.4 NM_177991.2 XM_017027654.1 XM_017027655.1 XM_017027656.1 XM_017027657.1 XM_017027658.1 XM_017027659.1 XM_017027660.1 XM_017027661.1 XM_017027662.1

Validation Results:

23%(+/-9% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00707875_s1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.