106199
NM_000691.4
NM_001135167.1
NM_001135168.1
XM_005256522.1
XM_005256523.2
XM_005256524.3
XM_011523730.1
XM_011523731.1
9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00167469_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.