SiRNA Validation Data

siRNA ID:

118

Gene ID:

1606

Transcript(s):

NM_001345.4 NM_201444.2 NM_201445.1 NM_201554.1 XM_005268688.2 XM_005268689.2 XM_005268690.2 XM_011537991.2 XM_011537993.2 XM_011537995.2 XM_017018900.1 XM_017018901.1 XM_017018902.1 XM_017018903.1 XM_017018904.1 XM_017018905.1 XM_017018906.1 XM_017018907.1 XM_017018908.1 XM_017018909.1

Validation Results:

25%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.