Close X

SiRNA Validation Data

siRNA ID:

118888

Gene ID:

31

Transcript(s):

NM_198834.2 NM_198836.2 NM_198837.1 NM_198838.1 NM_198839.2 XM_005257267.4 XM_006721853.1 XM_011524701.1 XM_011524703.1 XM_011524704.2 XM_017024553.1 XM_017024554.1 XM_017024555.1

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00167385_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.