s10252
NM_001278549.1
NM_002610.4
NR_103729.1
NR_103731.1
XM_006712594.2
XM_011511343.2
XM_011511344.2
XM_011511345.2
XM_011511347.2
20%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176853_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.