SiRNA Validation Data

siRNA ID:

s10715

Gene ID:

5371

Transcript(s):

NM_002675.3 NM_033238.2 NM_033239.2 NM_033240.2 NM_033244.3 NM_033246.2 NM_033247.2 NM_033249.2 NM_033250.2

Validation Results:

20%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00231241_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.