s11686
NM_001243718.1
NM_001243719.1
NM_002869.4
NM_198896.1
7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00818388_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.