SiRNA Validation Data

siRNA ID:

s17757

Gene ID:

9289

Transcript(s):

NM_001145770.2 NM_001145771.2 NM_001145772.2 NM_001145773.2 NM_001145774.2 NM_001290143.1 NM_001290144.1 NM_005682.6 NM_201524.3 NM_201525.3 XM_005256237.2 XM_005256238.2 XM_005256239.2 XM_005256240.4 XM_005256241.4 XM_005256242.4 XM_005256244.3 XM_005256245.2 XM_005256246.2 XM_005256247.2 XM_005256248.2 XM_005256249.3 XM_005256251.4 XM_005256252.2 XM_005256254.2 XM_005256255.2 XM_006721338.2 XM_006721339.3 XM_006721340.3 XM_006721341.2 XM_006721342.2 XM_006721343.3 XM_006721344.2 XM_006721345.3 XM_006721346.2 XM_006721347.2 XM_011523461.2 XM_011523462.2 XM_011523463.2 XM_011523464.2 XM_011523465.2 XM_011523466.2 XM_011523467.2 XM_011523468.2 XM_017023892.1

Validation Results:

4%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00173754_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.