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SiRNA Validation Data

siRNA ID:

s3097

Gene ID:

548596 1159

Transcript(s):

NM_001015001.2 NM_001321926.1 NM_001321927.1 NM_001321928.1 NM_001321929.1 NR_135856.1 XM_005254498.3 XM_017022369.1 XM_017022370.1 XM_017022371.1 NM_020990.4 NR_135748.1 NR_135749.1 NR_135750.1 NR_135751.1 XM_005254150.3 XM_011521194.1 XM_011521195.2 XM_011521196.1 XM_011521197.2 XM_011521198.1 XM_011521199.2 XM_017021902.1

Validation Results:

14%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00179727_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.