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SiRNA Validation Data

siRNA ID:

s34527

Gene ID:

64172

Transcript(s):

NM_022353.2 XM_005246766.3 XM_006712685.1 XM_006712686.1 XM_011511631.1 XM_017004676.1 XM_017004677.1 XM_017004678.1 XM_017004679.1 XM_017004680.1 XM_017004682.1 XM_017004683.1 XM_017004685.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00223547_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.