SiRNA Validation Data

siRNA ID:

s62

Gene ID:

3340

Transcript(s):

NM_001301063.1 NM_001543.4 XM_005268433.1 XM_005268434.2 XM_005268435.4 XM_005268436.3 XM_005268437.4 XM_005268438.1 XM_005268439.1 XM_005268442.3 XM_006714782.1 XM_006714783.1 XM_011537638.2 XM_017009427.1 XM_017009428.1 XM_017009429.1 XM_017009430.1 XM_017009431.1 XM_017009432.1 XM_017009433.1

Validation Results:

19%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00155454_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.