s7492
NM_000210.3
NM_001079818.2
NM_001316306.1
XM_006712510.1
XM_006712511.1
XM_017004005.1
XM_017004006.1
XM_017004007.1
XM_017004008.1
5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00173952_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.