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Product Details
100-65-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human,
Mouse,
Rat
Published species
Mouse
Expression System
CHO cells
Amino acid sequence
DSPSAPVNVT VRHLKANSAV VSWDVLEDEV VIGFAISQQK KDVRMLRFIQ EVNTTTRSCA LWDLEEDTEY IVHVQAISIQ GQSPASEPVL FKTPREAEKM ASKNKDEVTM KE
Molecular weight
25.2 kDa
Class
Recombinant
Type
Protein
Purity
≥ 90% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to promote cell survival and growth of rat H9c2 cardio-myoblasts under serum starvation conditions.
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Human/Murine/Rat Irisin, appears to form a non-disulfide linked oligomeric structure in solution, has a calculated theoretical molecular weight of 25.2 kDa, but migrates at an apparent molecular weight of 28 kDa by SDS-PAGE analysis under reducing conditions due to glycosylation.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; PeP; Peroxisomal protein
Gene Aliases: 1500001L03Rik; AI836596; C87088; FNDC5; FRCP2; irisin; PeP; Pxp
UniProt ID: (Human) Q8NAU1, (Rat) Q8K3V5, (Mouse) Q8K4Z2
Entrez Gene ID: (Human) 252995, (Rat) 260327, (Mouse) 384061
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