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Application
FIGURE: 1 / 1
Human CD200 His-tag Protein (A42571) in SDS-PAGE
SDS-PAGE analysis of Human CD200 His-tag (Product # A42571) shows purity to be: ≥ 98 % (SDS-PAGE), ≥ 90 % (SEC-HPLC).
Product Details
A42571
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD200, amino acidds Met1-Gly232 (Accession # NP_005935.4) with a C-terminal His-tag
Tag
His-tag
Molecular weight
24 kDa
Class
Recombinant
Type
Protein
Purity
≥98 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized Human CD200 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind CD200R Protein, Human, Recombinant (His & hFc Tag), the EC50 is 5-28 ng/mL.
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD200 is a type-1 membrane glycoprotein that contains two immunoglobulin domains, and belongs to the immunoglobulin superfamily. CD200 is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) and is implicated in locally preventing macrophage activation. Distant, but recognizable, homologues of CD200 have been identified in many herpesviruses and poxviruses. Studies of the related genes in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms of CD200 have been found. Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; OX-2 membrane glycoprotein
Gene Aliases: CD200; MOX1; MOX2; MRC; My033; OX-2
UniProt ID: (Human) B3KQI1
Entrez Gene ID: (Human) 4345
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