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Invitrogen
Human CDK1/cyclin A2, GST Tag Recombinant Protein
Product Details
PV6282
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
1-297
Tag
GST-tag
Molecular weight
60.4-74.8 kDa
Class
Recombinant
Type
Protein
Conjugate
Unconjugated
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 25% glycerol, 0.1mM EGTA, 0.1mM PMSF, 0.1mM EDTA, 150mM NaCl, 0.25mM DTT
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
The gene accession number for the subunits (CDK1/Cyclin A2) are NP_001777.1 and NP_001228.1.
Target Information
CDK1, or Cyclin-Dependent Kinase 1, is a critical member of the cyclin-dependent kinase family that regulates cell cycle progression. CDK1 is essential for the transition from the G2 phase to the M phase and for the progression through mitosis.
Cyclin A2 is a regulatory subunit that binds to CDK1 and activates its kinase activity. The binding of Cyclin A2 to CDK1 is necessary for the proper functioning of CDK1, as cyclins regulate the activation and specificity of CDKs.
Together, the CDK1/Cyclin A2 complex plays a vital role in the regulation of the cell cycle, particularly during the S phase and the G2/M transition. This complex is involved in the phosphorylation of key substrates required for DNA replication, the initiation of mitosis, and the proper alignment and separation of chromosomes, ensuring accurate and orderly cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; Cyclin A; Cyclin-A; Cyclin-A2; Cyclin-dependent kinase 1; p34 protein kinase
Gene Aliases: CCN1; CCNA; CCNA2; CDC2; CDC28A; CDK1; CDKN1; P34CDC2
UniProt ID: (Human) P20248, (Human) P06493
Entrez Gene ID: (Human) 890, (Human) 983
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