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Product Details
PV6382
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
CDK14: 1-469; Cyclin Y: 2-341
Tag
GST-tag
Molecular weight
77.0-65.5 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 0.1mM EDTA, 25% glycerol, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT, 10mM glutathione
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant human full-length CDK14 and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Target Information
CDK14 (PFTK1)/cyclin Y is a member of the CDC2-related protein kinase family which is expressed primarily in the postnatal and adult nervous system. CDK14 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. CDK14 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to CDK14 binding. CDK14 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of CDK14 predicts resistance to chemotherapy in patients with esophageal squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 14; Cyclin-dependent kinase 14; hPFTAIRE1; PFTAIRE protein kinase 1; Serine/threonine-protein kinase PFTAIRE-1
Gene Aliases: CDK14; KIAA0834; PFTAIRE1; PFTK1
UniProt ID: (Human) O94921
Entrez Gene ID: (Human) 5218
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