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Application
FIGURE: 1 / 1
Human CTLA-4 His-tag Protein (A42594) in SDS-PAGE
SDS-PAGE analysis of Human CTLA-4 His-tag (Product # A42594). The specification for purity shows: > 90 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).
Product Details
A42594
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CTLA4, amino acids Met1-Phe162 (Accession # NP_005205.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
15 kDa
Class
Recombinant
Type
Protein
Purity
>90 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Immobilized Recombinant Human CD86/B7-2 Protein (Fc Tag) at 2 µg/mL (100 µL/well) can bind Human CTLA-4 His-tag Recombinant Protein, the EC50 is 2.6-8 ng/mL.
2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80.
3.Captured Ipilimumab on proA Chip can bind Human CTLA-4 His-tag Recombinant Protein with an affinity constant of 20.03 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
4.Loaded Ipilimumab on ProA Biosensor, can bind Human CTLA-4 His-tag Recombinant Protein with an affinity constant of 5.15 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).
2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80.
3.Captured Ipilimumab on proA Chip can bind Human CTLA-4 His-tag Recombinant Protein with an affinity constant of 20.03 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
4.Loaded Ipilimumab on ProA Biosensor, can bind Human CTLA-4 His-tag Recombinant Protein with an affinity constant of 5.15 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD152; CD152 isoform; celiac disease 3; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; ICOS; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Gene Aliases: ALPS5; CD; CD152; CELIAC3; CTLA-4; CTLA4; GRD4; GSE; IDDM12
UniProt ID: (Human) P16410
Entrez Gene ID: (Human) 1493
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