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Product Details
RP-88048
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Met1-Ser645
Tag
His-tag
Molecular weight
69.76 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 µg/ml (100 µL/well) can bind Human EGFR His, the EC50 of Human EGFR His is 100-500 ng/mL.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Reconstituted with sterile deionized water to 0.5 mg/mL.
Concentration is measured by UV.
Target Information
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone
Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61
UniProt ID: (Human) P00533
Entrez Gene ID: (Human) 1956
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