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Invitrogen
Human IFN-alpha A (alpha 2a) (Carrier-Free) Recombinant Protein
Product Details
111012
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Molecular weight
19.2 kDa
Class
Recombinant
Type
Protein
Purity
>95%
Endotoxin concentration
<1 EU/µg
Activity
EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alpha-A) provided by the National Institutes of Health [see Pestka, S. (1986). Methods in Enzymology, Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
After receipt, the product may be stored at -20°C for short-term use (less than 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.
Target Information
Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-2a interferon; IFN-alpha-2; Interferon; interferon alpha 2a; interferon alpha 2b; interferon alpha A; Interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A
Gene Aliases: IFN-alphaA; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; INFA2
UniProt ID: (Human) P01563
Entrez Gene ID: (Human) 3440
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