Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PHC9614
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Tag
His-tag
Molecular weight
26 kDa
Class
Recombinant
Type
Protein
Purity
≥95% by SDS-PAGE
Endotoxin concentration
<1 EU/µg
Activity
ED50 ≤ 0.2 µg/mL; determined by the dose-dependent inhibition of MCF-7 cell proliferation in the presence of IGF2 (14 ng/mL).
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged to bring the contents to the bottom prior to opening. Lyophilized recombinant human IGFBP-4 should be reconstituted in sterile PBS to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at -20°C. Further dilutions should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
Storage: Lyophilized human IGFBP-4 should be stored at 2-8°C, preferably desiccated. Store reconstituted human IGFBP-4 at -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
Target Information
This gene is a member of the insulin-like growth factor binding protein family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: IBP-4; IGF-binding protein 4; IGFBP; Insulin like growth factor binding protein; insulin-like growth factor binding protein 4; Insulin-like growth factor-binding protein 4
Gene Aliases: BP-4; HT29-IGFBP; IBP4; IGFBP-4; IGFBP4
UniProt ID: (Human) P22692
Entrez Gene ID: (Human) 3487
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support