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Product Details
RP-10908
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Not Applicable
Expression System
E. coli
Amino acid sequence
Human Insulin is a two chain, non-glycosylated polypeptide containing 51 amino acids.
Molecular weight
5.8 kDa
Class
Recombinant
Type
Protein
Activity
Fully biologically active as compared to WHO reference standard at 28 U/mg.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Purification
purified
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This is a recombinant protein suitable for use as a control in ELISA and Western blot applications.
Lyophilized Insulin is stable at room temperature for 3 weeks. Reconstitute in sterile 0.005N HCl to a concentration of no more than 1mg/mL. Store lyophilized product at -20ºC. Upon reconstitution, store at 4ºC for 2-7 days or aliquot and store at -20ºC. For long term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.
Target Information
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ins; Insulin; preproinsulin; proinsulin
Gene Aliases: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10
UniProt ID: (Human) P01308
Entrez Gene ID: (Human) 3630
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