Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
RP-88227
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse CTLA4, amino acids Met1-Phe162 (Accession # NP_033973.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
25-30 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Immobilized recombinant mouse CTLA4-His at 2 µg/ml (100 µL/well) can mouse CD80-Fc . The EC50 of mouse CD80-Fc is 1-3 ng/mL.
2. Measured by its ability to inhibit IL-2 secretion by stim µLated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80.
2. Measured by its ability to inhibit IL-2 secretion by stim µLated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Reconstituted with sterile deionized water to 0.25mg/mL.
Concentration is measured by UV.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD152; CD152 antigen; CTLA-4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; ICOS
Gene Aliases: Cd152; Ctla-4; Ctla4; Ly-56
UniProt ID: (Mouse) P09793
Entrez Gene ID: (Mouse) 12477
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support