Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
FIGURE: 1 / 1
Mouse EphA3 Fc Chimera Protein (RP-88231) in SDS-PAGE
SDS-PAGE analysis of Mouse EphA3 Fc Chimera (Product # RP-88231) shows purity to be: > 90 % (SDS-PAGE).
Product Details
RP-88231
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse EPHA3, amino acids Met1-His541 (Accession # NP_034270.1) with a C-terminal human IgG1 Fc region
Tag
Fc-tag
Molecular weight
96 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10 µg/ml (100 µL/well) can bind mouse EPHA3-Fc, The EC50 of rat mouse EPHA3-Fc is 12.3-28.9 ng/mL.
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (800 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
EPHA3 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA3 encodes a protein that binds ephrin-A ligands and receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: EK4; EPH-like kinase 4; ephrin type-A receptor 3; H TYRO4; tyrosine-protein kinase receptor ETK1; tyrosine-protein kinase TYRO4
Gene Aliases: AW492086; Cek4; End3; ETK1; Hek; Hek4; Mek4; Tyro4
Entrez Gene ID: (Mouse) 13837
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support