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Application
FIGURE: 1 / 1
PeproGMP® Human TPO (Thrombopoietin) Protein (GMP300-18-50UG) in Functional
Bioassay of PeproGMP® Human TPO Recombinant Protein, PeproTech (Product # GMP300-18-50UG) compared to Human TPO, Animal-Free Recombinant Protein, PeproTech (Product # 300-18-10UG">AF-300-18-10UG) and Human TPO Recombinant Protein, PeproTech (Product # 300-18-10UG), demonstrating comparable bioactivity across product grades.
Product Details
GMP300-18-50UG
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Molecular weight
18.6 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<0.1 EU/µg
Activity
Determined by its ability to stimulate human MO7e cell proliferation. PeproGMP Recombinant HumanTPO has been tested against the WHO standard NIBSC 03/124. Find the International Unit (IU) value here.
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Thrombopoietin (TPO) is a hematopoietic growth factor that stimulates the proliferation and maturation of megakaryocytes. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. PeproTech Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
PeproGMP TPO cytokine is most often used with PeproGMP SCF and PeproGMP Flt3-ligand in ex-vivo cell therapy for the proliferation of hematopoietic stem cell (HSC) cultures, along with IL-3 and IL-6 for further synergistic expansion and immune cell maturation.
Additional product information , specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Bioinformatics
Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; ML; MPL ligand; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; Thrombopoietin
Gene Aliases: MGDF; MKCSF; ML; MPLLG; THCYT1; THPO; TPO
UniProt ID: (Human) P40225
Entrez Gene ID: (Human) 7066
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